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61.
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Abstract. Since the construction in 1979 of a dam in the Logone floodplain in the Sahelo‐Sudanian zone of Cameroon, annual inundations have decreased, reducing perennial vegetation as important grazing source for nomadic herds and wildlife during the dry season. Presently, possibilities exist to release excess water for floodplain rehabilitation. In 1994 a pilot release was executed, reflooding 200 km2, to verify predicted advantages. Vegetation has been studied from 1984 onwards along a transect covering flooded, recently reflooded and desiccated parts of the floodplain. Since 1993, the floristic composition has also been monitored in a grid in the centre of the impact zone. Cover of perennial grasses, most notably Echinochloa pyr amidalis and Oryza longistaminata increased from 41 to 61% in the reflooded zone. Vetiveria nigritana, a tussock grass that used to be dominant, disappeared slowly after the dam construction and has not shown a comeback. The cover of annual species, most notably Sorghum arundinaceum, a dominant annual grass only since the mid‐1980s, decreased in the reflooded zone from 58% to 34%. If the present conversion rate of annual into perennial grassland is extrapolated, recovery towards a 100% perennial state may be reached after the 2003 flooding season. Apart from favourable climatic conditions, recovery might be dependent on the restoration of soil fertility, limiting an approach focusing on flooding depth only. 相似文献
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BackgroundAn effective, green and rapid analytical strategy namely the simultaneous spray assisted droplet formation-liquid phase microextraction (S-SADF-LPME) method was developed for the determination of trace quantity of cadmium in saliva samples by using the slotted quartz tube-flame atomic absorption spectrophotometry (SQT-FAAS). By the developed method, external dispersive solvent usage for droplet formation was reduced to obtain a more environmental-friendly method.MethodsMethod consists of a simultaneous complexing and extraction step, which was based on spraying an extraction solvent containing a solid ligand into the aqueous sample solution, forming fine droplets without the use of dispersive solvent. The procedure was implemented using a customized, cost effective and portable spray apparatus to minimize the consumption of reagent, analysis time and operation steps. Thus, this methodology ensures better repeatability and accuracy while minimizing the relative errors caused by the experimental steps. Parameters including the buffer amount, extractant/ligand concentration, extraction solvent type, extraction/ligand solution volume, spraying number and vortex period were systemically optimized to lower the detection limit.ResultsUnder the optimal extraction conditions, 96.9-folds enhancement in the detection power of the traditional FAAS was achieved. The limit of detection and limit of quantification values of presented method were calculated to be 0.65 and 2.17 ng mL−1, respectively. Accuracy and applicability of the optimized method was investigated by collecting saliva samples from smokers. Satisfactory percent recovery values wereachieved for cadmium with a low standard deviation in the acceptable range of 84.9–109.6 %.ConclusionThe developed dispersive solvent-free S-SADF-LPME technique presents a fast, simple, cost-effective and eco-friendly microextraction method based on the use of an easily accessible and functional spray apparatus. 相似文献
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Summary We previously introduced a flash spectrophotometric method to analyze proton conduction by CF0 in vesicles derived from thylakoid membranes (H. Lill, S. Engelbrecht, G. Schönknecht & W. Junge, 1986,Eur. J. Biochem.
160:627–634). The unit conductance of CF0, as revealed by this technique, was orders of magnitude higher than that theoretically expected for a hydrogen-bonded chain. We scrutinized the validity of this method. Small vesicles were derived from thylakoids by EDTA treatment. The intrinsic electric generators in the membrane were stimulated by short flashes of light and the relaxation of the voltage via ionic channels was measured through electrochromic absorption changes of intrinsic pigments. The voltage decay was stimulated by a statistical model. As the vesicle-size distribution had only a minor influence, the simulation required only two fit parameters, the first proportional to the unit conductance of an active channelG, and the second denoting the average number of active channels per vesiclen. This technique was applied to CF0, the proton channel of the chloroplast ATP synthase, and to gramicidin, serving as a standard. For both channels we found the above two fit parameters physically meaningful. They could be independently varied in predictable wasy, i.e.n by addition of known inhibitors of F0-type proton channels andG via the temperature. for gramicidin, the unit conductance (2.7 pS) was within the range described in the literature. This established the competence of this method for studies on the mechanism of proton conduction by CF0, whose conductance so far has not been accessible to other, more conventional approaches. The time-averaged unit conductance of CF0 was about 1 pS, equivalent to the turnover of 6×105 H+/(CF0·sec) at 100 mV driving force. 相似文献
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合欢皮与山合欢皮的理化性质的比较研究 总被引:1,自引:0,他引:1
采用紫外分光光度法,薄层色谱法,溶血试验,泡沫反应等,对合欢皮与山合欢皮的理化性质进行了比较研究。结果表明:可以将山合欢皮作合欢皮使用。 相似文献
69.
本试验通过对高氯酸和硫酸体系进行比较,选择香草醛-冰醋酸-高氯酸体系作为测定苦瓜皂甙的合适显色条件,并对体系中各个因素逐一考察,确定比色法测定苦瓜皂甙的条件为:0.2mL冰醋酸,0.8mL高氯酸,5%香草醛.冰醋酸溶液,70℃条件下反应20min。试验以苦瓜皂甙建立了苦瓜皂甙测定回归曲线,线性和稳定性可靠,平均加标回收率100.78%,变异系数为1.910%。 相似文献
70.
虎杖营养器官蒽醌类化合物含量的季节变化 总被引:10,自引:0,他引:10
采用差示分光光度法测定虎杖营养器官中蒽醌类化合物的含量.该法以醋酸镁甲醇液为显色剂,以大黄素作标准对照品.其回归方程y=0.0576x+0.0012,r=0.9962.结果表明,在一个生长期中,各营养器官总蒽醌的变化不明显,蒽醌类化合物在各营养器官中含量的顺序依次为根>叶>茎;三年生的根高于一年生根;幼嫩茎叶的含量高于成熟的茎叶.在三年生根中游离蒽醌的含量在7月和9月出现两个峰值,8月降至最低;一年生根、幼叶、幼茎和老茎中游离蒽醌的含量在7月左右最高;除根外,幼叶的蒽醌类化合物的含量较其它营养器官高,且其生物量大,因此幼叶具有一定的开发前景. 相似文献